Amidating enzyme

PAM activity is detected in serum, but its significance and utility as a clinical biomarker remain unexplored.Methods: We used well-established enzymatic assays specific for the peptidylglycine-α -hydroxylating monooxygenase (PHM) and peptidyl-α-hydroxyglycine α-amidating lyase (PAL) domains of PAM to quantify amidating activity in the sera of 144 elderly men.

The first enzyme activity, peptidylglycine α-hydroxylating monooxygenase, catalyzes the oxygen-, ascorbate-, and copper-dependent formation of α-hydroxyglycine derivatives.Relationships between PHM and PAL activity and serum levels of their respective active-site metals, Cu and Zn, were analyzed.Study participants were also genotyped for eight non-coding single nucleotide polymorphisms (SNPs) in PAM, and relationships between genotype and serum enzyme activity and metal levels were analyzed.This page highlights the latest research on the design and application of Custom Synthetic Peptides in science, medicine, and technology.Gen Script is proud to be part of the discovery research taking place all around the globe.

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